Micronuclei assay by laser scanning cytometry
نویسندگان
چکیده
منابع مشابه
Automation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry.
Here we describe the adaptation of laser scanning cytometry (LSC) to measure micronuclei (MN) automatically in lymphocytes. MN frequencies were determined in irradiated human lymphocytes using the cytokinesis-block technique, and the results from LSC were compared with visual scoring results obtained from slides of cells stained using Fast Green and 4',6-diamidino-2-phenylindole (DAPI). This fl...
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Laser scanning cytometry (LSC) generates quantitative information on immune receptor expression from cells cytocentrifuged onto a microscope slide. In children, the description of developmental changes in immune receptor expression on alveolar macrophages (AM) has been limited by the small number of cells recovered by bronchoalveolar lavage (BAL). The applicability of LSC to the study of AM fro...
متن کاملAn automated microfluidic sample preparation system for laser scanning cytometry.
Laser scanning cytometry (LSC) is emerging as a clinical tool. In one application a "Clatch" slide, named after the inventor, is used in conjunction with LSC for cell surface marker immunophenotyping of patient samples. The slide requires time consuming and laborious pipetting steps, making a test tedious and prone to handling errors. The Clatch slide also uses a significant number of cells, li...
متن کاملApplication of Laser Scanning Cytometry to Clinical and Laboratory Hematology
Morphological diagnosis by May-Gruenwald-Giemsa (MGG) staining is an essential method in clinical and laboratory hematology. In addition, the detection of chromosomal aberration by G-band methods, the phenotyping of surface markers by Flowcytometry (FCM) or genetic diagnosis by reverse transcriptase-polymerase chain reaction (RT-PCR), Fluorescence in situ hybridization (FISH) and Southern blott...
متن کاملAnalysis of Ploidy in Hypopharyngeal Cancer by Laser Scanning Cytometry on Fine Needle Aspirate Biopsies
AIM To test laser scanning cytometry (LSC) for the analysis of ploidy in squamous cell carcinoma of the hypopharynx (SCCH) and to develop a routine application for minimal samples such as fine needle aspirate biopsies (FNABs). METHODS From 11 individuals 30 FNABs of primary tumors (n=11) and lymphatic metastases of SCCH (n=11) and non-metastatic lymph nodes (n=8) are analyzed by LSC. This mic...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2001
ISSN: 0196-4763,1097-0320
DOI: 10.1002/1097-0320(20010901)45:1<19::aid-cyto1140>3.0.co;2-g